Review



grk3  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 91
      Buy from Supplier

    Structured Review

    Addgene inc grk3
    Fig. 1. GRK2 and <t>GRK3,</t> but not GRK5 or GRK6, rapidly desensitize group I mGluR-mediated Ca2+ responses. (A) Representative normalized average traces showing kinetics of glutamate-induced R-GECO response to mGluR1 activation. (B) Summary bar graph showing effects of GRK coexpression on FWHM. (C) Zoom-in to represent ative traces showing the effect of GRK2 on ON kinetics of R-GECO response. (D) Summary bar graph showing the effects of GRK coexpression on rise time. (E and F) Same as (A) and (B), but for mGluR5. (G) Representative individual cell traces showing oscillatory responses, which are reduced with GRK2 coexpression. (H) Summary bar graph showing the effects of GRKs on the proportion of cells showing oscillatory responses to glutamate. (I) Representative normalized average traces showing kinetics of glutamate-induced R-GECO response to mGluR1 activation in parental versus GRK2/3 double KO cells. (J) Summary bar graph showing the effects of GRK2/3 double KO and GRK2 rescue on FWHM. (K) Summary histogram showing the distribution of FWHM values across individual cells for each condition and replicate in (J). Fifty to 80 cells per experiment are used to produce a normalized histogram that is averaged across separate experiments to produce mean histograms with error bars. GRK2/3 KO pro- duces a shift in the distribution toward higher FWHM values, while GRK2 rescue produces dose-dependent shifts toward lower values and a narrower distribution. In bar graphs, each point represents an independent measurement; all graphs come from two to five separate experimental days, and error bars represent SEM. One-way ANOVA with Tukey’s multiple comparisons tests. *P < 0.05, **P < 0.01, and ***P < 0.001.
    Grk3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/grk3/product/Addgene inc
    Average 91 stars, based on 3 article reviews
    grk3 - by Bioz Stars, 2026-02
    91/100 stars

    Images

    1) Product Images from "Control of Gα q signaling dynamics and GPCR cross-talk by GRKs."

    Article Title: Control of Gα q signaling dynamics and GPCR cross-talk by GRKs.

    Journal: Science advances

    doi: 10.1126/sciadv.abq3363

    Fig. 1. GRK2 and GRK3, but not GRK5 or GRK6, rapidly desensitize group I mGluR-mediated Ca2+ responses. (A) Representative normalized average traces showing kinetics of glutamate-induced R-GECO response to mGluR1 activation. (B) Summary bar graph showing effects of GRK coexpression on FWHM. (C) Zoom-in to represent ative traces showing the effect of GRK2 on ON kinetics of R-GECO response. (D) Summary bar graph showing the effects of GRK coexpression on rise time. (E and F) Same as (A) and (B), but for mGluR5. (G) Representative individual cell traces showing oscillatory responses, which are reduced with GRK2 coexpression. (H) Summary bar graph showing the effects of GRKs on the proportion of cells showing oscillatory responses to glutamate. (I) Representative normalized average traces showing kinetics of glutamate-induced R-GECO response to mGluR1 activation in parental versus GRK2/3 double KO cells. (J) Summary bar graph showing the effects of GRK2/3 double KO and GRK2 rescue on FWHM. (K) Summary histogram showing the distribution of FWHM values across individual cells for each condition and replicate in (J). Fifty to 80 cells per experiment are used to produce a normalized histogram that is averaged across separate experiments to produce mean histograms with error bars. GRK2/3 KO pro- duces a shift in the distribution toward higher FWHM values, while GRK2 rescue produces dose-dependent shifts toward lower values and a narrower distribution. In bar graphs, each point represents an independent measurement; all graphs come from two to five separate experimental days, and error bars represent SEM. One-way ANOVA with Tukey’s multiple comparisons tests. *P < 0.05, **P < 0.01, and ***P < 0.001.
    Figure Legend Snippet: Fig. 1. GRK2 and GRK3, but not GRK5 or GRK6, rapidly desensitize group I mGluR-mediated Ca2+ responses. (A) Representative normalized average traces showing kinetics of glutamate-induced R-GECO response to mGluR1 activation. (B) Summary bar graph showing effects of GRK coexpression on FWHM. (C) Zoom-in to represent ative traces showing the effect of GRK2 on ON kinetics of R-GECO response. (D) Summary bar graph showing the effects of GRK coexpression on rise time. (E and F) Same as (A) and (B), but for mGluR5. (G) Representative individual cell traces showing oscillatory responses, which are reduced with GRK2 coexpression. (H) Summary bar graph showing the effects of GRKs on the proportion of cells showing oscillatory responses to glutamate. (I) Representative normalized average traces showing kinetics of glutamate-induced R-GECO response to mGluR1 activation in parental versus GRK2/3 double KO cells. (J) Summary bar graph showing the effects of GRK2/3 double KO and GRK2 rescue on FWHM. (K) Summary histogram showing the distribution of FWHM values across individual cells for each condition and replicate in (J). Fifty to 80 cells per experiment are used to produce a normalized histogram that is averaged across separate experiments to produce mean histograms with error bars. GRK2/3 KO pro- duces a shift in the distribution toward higher FWHM values, while GRK2 rescue produces dose-dependent shifts toward lower values and a narrower distribution. In bar graphs, each point represents an independent measurement; all graphs come from two to five separate experimental days, and error bars represent SEM. One-way ANOVA with Tukey’s multiple comparisons tests. *P < 0.05, **P < 0.01, and ***P < 0.001.

    Techniques Used: Activation Assay



    Similar Products

    grk3  (Addgene inc)
    91
    Addgene inc grk3
    Fig. 1. GRK2 and <t>GRK3,</t> but not GRK5 or GRK6, rapidly desensitize group I mGluR-mediated Ca2+ responses. (A) Representative normalized average traces showing kinetics of glutamate-induced R-GECO response to mGluR1 activation. (B) Summary bar graph showing effects of GRK coexpression on FWHM. (C) Zoom-in to represent ative traces showing the effect of GRK2 on ON kinetics of R-GECO response. (D) Summary bar graph showing the effects of GRK coexpression on rise time. (E and F) Same as (A) and (B), but for mGluR5. (G) Representative individual cell traces showing oscillatory responses, which are reduced with GRK2 coexpression. (H) Summary bar graph showing the effects of GRKs on the proportion of cells showing oscillatory responses to glutamate. (I) Representative normalized average traces showing kinetics of glutamate-induced R-GECO response to mGluR1 activation in parental versus GRK2/3 double KO cells. (J) Summary bar graph showing the effects of GRK2/3 double KO and GRK2 rescue on FWHM. (K) Summary histogram showing the distribution of FWHM values across individual cells for each condition and replicate in (J). Fifty to 80 cells per experiment are used to produce a normalized histogram that is averaged across separate experiments to produce mean histograms with error bars. GRK2/3 KO pro- duces a shift in the distribution toward higher FWHM values, while GRK2 rescue produces dose-dependent shifts toward lower values and a narrower distribution. In bar graphs, each point represents an independent measurement; all graphs come from two to five separate experimental days, and error bars represent SEM. One-way ANOVA with Tukey’s multiple comparisons tests. *P < 0.05, **P < 0.01, and ***P < 0.001.
    Grk3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/grk3/product/Addgene inc
    Average 91 stars, based on 1 article reviews
    grk3 - by Bioz Stars, 2026-02
    91/100 stars
    		  Buy from Supplier
    pcdna grk3  (Addgene inc)
    91
    Addgene inc pcdna grk3
    Fig. 1. GRK2 and <t>GRK3,</t> but not GRK5 or GRK6, rapidly desensitize group I mGluR-mediated Ca2+ responses. (A) Representative normalized average traces showing kinetics of glutamate-induced R-GECO response to mGluR1 activation. (B) Summary bar graph showing effects of GRK coexpression on FWHM. (C) Zoom-in to represent ative traces showing the effect of GRK2 on ON kinetics of R-GECO response. (D) Summary bar graph showing the effects of GRK coexpression on rise time. (E and F) Same as (A) and (B), but for mGluR5. (G) Representative individual cell traces showing oscillatory responses, which are reduced with GRK2 coexpression. (H) Summary bar graph showing the effects of GRKs on the proportion of cells showing oscillatory responses to glutamate. (I) Representative normalized average traces showing kinetics of glutamate-induced R-GECO response to mGluR1 activation in parental versus GRK2/3 double KO cells. (J) Summary bar graph showing the effects of GRK2/3 double KO and GRK2 rescue on FWHM. (K) Summary histogram showing the distribution of FWHM values across individual cells for each condition and replicate in (J). Fifty to 80 cells per experiment are used to produce a normalized histogram that is averaged across separate experiments to produce mean histograms with error bars. GRK2/3 KO pro- duces a shift in the distribution toward higher FWHM values, while GRK2 rescue produces dose-dependent shifts toward lower values and a narrower distribution. In bar graphs, each point represents an independent measurement; all graphs come from two to five separate experimental days, and error bars represent SEM. One-way ANOVA with Tukey’s multiple comparisons tests. *P < 0.05, **P < 0.01, and ***P < 0.001.
    Pcdna Grk3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcdna grk3/product/Addgene inc
    Average 91 stars, based on 1 article reviews
    pcdna grk3 - by Bioz Stars, 2026-02
    91/100 stars
    		  Buy from Supplier

    Image Search Results


    Fig. 1. GRK2 and GRK3, but not GRK5 or GRK6, rapidly desensitize group I mGluR-mediated Ca2+ responses. (A) Representative normalized average traces showing kinetics of glutamate-induced R-GECO response to mGluR1 activation. (B) Summary bar graph showing effects of GRK coexpression on FWHM. (C) Zoom-in to represent ative traces showing the effect of GRK2 on ON kinetics of R-GECO response. (D) Summary bar graph showing the effects of GRK coexpression on rise time. (E and F) Same as (A) and (B), but for mGluR5. (G) Representative individual cell traces showing oscillatory responses, which are reduced with GRK2 coexpression. (H) Summary bar graph showing the effects of GRKs on the proportion of cells showing oscillatory responses to glutamate. (I) Representative normalized average traces showing kinetics of glutamate-induced R-GECO response to mGluR1 activation in parental versus GRK2/3 double KO cells. (J) Summary bar graph showing the effects of GRK2/3 double KO and GRK2 rescue on FWHM. (K) Summary histogram showing the distribution of FWHM values across individual cells for each condition and replicate in (J). Fifty to 80 cells per experiment are used to produce a normalized histogram that is averaged across separate experiments to produce mean histograms with error bars. GRK2/3 KO pro- duces a shift in the distribution toward higher FWHM values, while GRK2 rescue produces dose-dependent shifts toward lower values and a narrower distribution. In bar graphs, each point represents an independent measurement; all graphs come from two to five separate experimental days, and error bars represent SEM. One-way ANOVA with Tukey’s multiple comparisons tests. *P < 0.05, **P < 0.01, and ***P < 0.001.

    Journal: Science advances

    Article Title: Control of Gα q signaling dynamics and GPCR cross-talk by GRKs.

    doi: 10.1126/sciadv.abq3363

    Figure Lengend Snippet: Fig. 1. GRK2 and GRK3, but not GRK5 or GRK6, rapidly desensitize group I mGluR-mediated Ca2+ responses. (A) Representative normalized average traces showing kinetics of glutamate-induced R-GECO response to mGluR1 activation. (B) Summary bar graph showing effects of GRK coexpression on FWHM. (C) Zoom-in to represent ative traces showing the effect of GRK2 on ON kinetics of R-GECO response. (D) Summary bar graph showing the effects of GRK coexpression on rise time. (E and F) Same as (A) and (B), but for mGluR5. (G) Representative individual cell traces showing oscillatory responses, which are reduced with GRK2 coexpression. (H) Summary bar graph showing the effects of GRKs on the proportion of cells showing oscillatory responses to glutamate. (I) Representative normalized average traces showing kinetics of glutamate-induced R-GECO response to mGluR1 activation in parental versus GRK2/3 double KO cells. (J) Summary bar graph showing the effects of GRK2/3 double KO and GRK2 rescue on FWHM. (K) Summary histogram showing the distribution of FWHM values across individual cells for each condition and replicate in (J). Fifty to 80 cells per experiment are used to produce a normalized histogram that is averaged across separate experiments to produce mean histograms with error bars. GRK2/3 KO pro- duces a shift in the distribution toward higher FWHM values, while GRK2 rescue produces dose-dependent shifts toward lower values and a narrower distribution. In bar graphs, each point represents an independent measurement; all graphs come from two to five separate experimental days, and error bars represent SEM. One-way ANOVA with Tukey’s multiple comparisons tests. *P < 0.05, **P < 0.01, and ***P < 0.001.

    Article Snippet: The following cDNA for bovine GRKs were used: untagged GRK2 (Addgene #14691), GRK3 (Addgene #32689), GRK5 (Addgene #14690), GRK6 (Addgene #32693), GRK2-GFP (24), and GRK2-CAAX (Addgene #166224).

    Techniques: Activation Assay